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. Author manuscript; available in PMC: 2022 Feb 15.
Published in final edited form as: Cell Rep. 2021 Oct 12;37(2):109813. doi: 10.1016/j.celrep.2021.109813

Figure 6. DNA damage and senescence markers are increased in dysfunctional male MafAS64F/+ islets.

Figure 6.

(A–C) gH2AX staining (A) and 53BP1 (B), markers of DNA double-strand breaks, and p21, a cell-cycle inhibitor (C), were present in male MafAS64F/+ (Het) islets. Male MafAS64F/+ islets had a significant increase in the proportion of islets with >10% p21+ cells.

(D) Male MafAS64F/+ islets showed reduced LaminB1 protein (left) and mRNA (right).

(E and F) SA-β-gal was not produced in 4-week-old male MafAS64F/+ islets, but was detected in MafAS64F/+ males by 7 weeks. (E) The SA-β-gal in 7-week-old MafAS64F/+ islets was of similar intensity to 10- to 12-month-old WT male mouse islets. (F) The proportion of SA-β-gal+ islets was quantitated for each sample (n = 3–4). Two-tailed Student t test; **p < 0.01; scale bar, 50 μm.