Figure 2.

PDGF-induced upregulation of Plk1–Casp9/3 pathway is PDGF receptor dependent. HASM cells were treated with 10 ng/ml PDGF for 24 hours in the presence or absence of 10 μM AG1296. Dimethyl sulfoxide (DMSO) was used as vehicle. (B–F) Protein ratios of Plk1/GAPDH (B), Casp9 phosphorylation at Ser-196 (C), Casp9 phosphorylation at Thr-125 (D), pro-Casp9 concentration (E), and pro-Casp3 concentration (F) are normalized to Ctrl cells not treated with AG1296, respectively. Data are mean values of five to six nonasthmatic cell cultures from three donors. Error bars indicate SD. *P < 0.05 and **P < 0.01. Two-way ANOVA was used for statistical analysis.