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. 2021 Oct 27;66(2):223–234. doi: 10.1165/rcmb.2021-0192OC

Figure 5.

Figure 5.

Knockdown (KD) of Plk1 affects Casp9 phosphorylation at Ser-196 and concentrations of Casp9/3 in HASM cells. (A) Representative IBs illustrating the effects of Plk1 KD on the levels of Casp9 phosphorylation at Ser-196, pro-Casp9, and pro-Casp3. Ctrl and Plk1 KD cells were treated with or without 10 ng/ml PDGF for 24 hours, followed by IB analysis. (B) Protein ratios of Plk1/GAPDH are normalized to cells treated with Ctrl siRNA. Plk1 KD is verified in these cells. (C–E) Plk1 KD reduces Casp9 phosphorylation at Ser-196 (C) and the concentrations of pro-Casp9 (D) and pro-Casp3 (E). (F) Apoptosis of HASM cells was evaluated using the Annexin V apoptosis assay kit. Data are mean values of four to six nonasthmatic cell cultures from three donors. Error bars indicate SD. *P < 0.05 and **P < 0.01. Two-way ANOVA was used for statistical analysis.