Table 2.
Summary of CRISPR/Cas9-induced mutations in T0 plants
Construct | # of sequenced plants | # of plants verified for transformation | # of plants with mutations in GmPDS18g | # of plants with mutations in GmPDS11g | Mutation efficiency (%) | Indel frequency (%) |
---|---|---|---|---|---|---|
GmPDS1 | 2 | 2 | 2 | 0 | 100 | 6.5–16 |
GmPDS3 | 8 | 5 | 5 | 0 | 100 | 8–88 |
GmPDS7 | 4 | 4 | 0 | 3 | 75 | 9–21 |
GmPDS8 | 11 | 10 | 9 | 9 | 90 | 3–100 |
GmPDS9 | 6 | 5 | 4 | 5 | 90 | 4–100 |
Mutation efficiency (%) is calculated as number of plants showing mutations divided by number of plants verified for transformation
Indel frequency (%) is calculated as number of clones showing mutations divided by total number of sequenced clones