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. Author manuscript; available in PMC: 2022 Feb 15.
Published in final edited form as: Cell Rep. 2022 Jan 18;38(3):110278. doi: 10.1016/j.celrep.2021.110278

Figure 4. PSAT1 expression predicts sensitivity to serine starvation in patient-derived ER+ breast cancer organoids.

Figure 4.

(A) Clinical characteristics of the 6 patient-derived organoid (PDO) models.

(B) Western blot for ERα and PSAT1 in PDO models. HCC1806 basal breast cancer cell lysate is included as an ER−/PSAT1 high reference.

(C) Representative confocal images of DAPI (blue) and EdU (pink) staining in PDO models treated with or without S/G for 10 days.

(D) Quantification of EdU staining in PDO models. %EdU+ cells were calculated by dividing the number of EdU+ cells by the total number of cells in either 16 or 25 organoids per group. p values are from unpaired Student’s t tests.