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. 2022 Feb 15;20:80. doi: 10.1186/s12951-022-01287-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — In vivo activation of immune responses triggered by PTT/SDT. A The DC maturation levels induced by CHINPs-based PTT/STT on 4T1 tumor-bearing mice (gated on CD11c⁺ DC cells) analyzed by flow cytometry and B the corresponding quantitative analysis. C The CD8⁺ T cells (CD3⁺CD8⁺) analyzed by flow cytometry and D the corresponding quantitative analysis. E The Treg cells (CD3⁺CD4⁺Foxp3⁺) analyzed by flow cytometry and F the corresponding quantitative analysis. G–I Cytokine levels in sera from mice isolated at 7d post different treatments. Data shown are mean ± SD (n = 3). Statistical differences determined by one-way ANOVA; *p < 0.05, **p < 0.01, ***p < 0.0001, ****p < 0.0001