Fig. 4.

Circ_IRAK3 exerted a role in BC via miR-603. A–K HCC70 and MDA-MB-231 cells were transfected with si-NC, si-circ_IRAK3, si-circ_IRAK3 + anti-miR-NC, or si-circ_IRAK3 + anti-miR-603. A Expression of miR-603 in HCC70 and MDA-MB-231 cells was surveyed by qRT-PCR. B–F Flow cytometry, colony formation, and MTT assays were performed to evaluate the cell cycle progression, colony formation, or proliferation of HCC70 and MDA-MB-231 cells. G–I Transwell and flow cytometry assays were conducted to analyze cell migration, invasion, or apoptosis in HCC70 and MDA-MB-231 cells. J, K Western blotting revealed the levels of Bax, Cleaved PARP, and Bcl-2 in HCC70 and MDA-MB-231 cells. *P < 0.05