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. Author manuscript; available in PMC: 2022 Feb 15.
Published in final edited form as: Bioconjug Chem. 2021 May 19;32(6):1156–1166. doi: 10.1021/acs.bioconjchem.1c00220

Figure 3:

Figure 3:

Adding docking strand (DS) to antibody-linker. (A) Separating free DS using 100 kDa MWCO centrifugal filters. The DS and antibody-linker conjugate are incubated, then the sample is added to the molecular weight cut-off filters. (B) Expected separation of components after spin and wash steps. (C) Retentate absorbances at 260nm. Results show an increased signal at 260nm when the DS is in the presence of the antibody-linker conjugate. An increased signal can also be seen for the case of just the DS and antibody, which is accounted for in later steps.