Skip to main content
. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Hypertension. 2021 Nov 17;79(1):126–138. doi: 10.1161/HYPERTENSIONAHA.121.17672

Figure 2. Cholesterol insensitive mutant of Kir2.1 recovers the cholesterol effect on impaired FIV in hypercholesterolemic mouse resistance arteries.

Figure 2.

A. (Left) Representative traces of Kir2.1 currents in freshly isolated mesenteric ECs from WT, ApoE−/−, Kir2.1L222I, and ApoE−/−Kir2.1L222I mice. (Right) Average Kir current densities (WT:n=16 cells from 4 mice, 11.3±1.5pF, Kir2.1L222I:n=19 cells from 4 mice, 14.57±1.02pF, ApoE−/−:n=14 cells from 4 mice, 12.6±2.2pF, ApoE−/−Kir2.1L222I: n=14 cells from 4 mice, 12.5±1.2pF. *p<0.05 ***p < 0.005, ****p < 0.001). B. (Left) Representative traces of potassium currents of Kir2.1 recorded in freshly isolated mesenteric ECs from WT, ApoE−/−, Kir2.1L222I and ApoE−/−Kir2.1L222I mice with and without shear-stress. (Right) Average Kir current densities (WT:n=7 cells from 4 mice, 11.8±4.0pF, Kir2.1L222I:n=6 cells from 4 mice, 9.7±1.9pF, ApoE−/−:n=6 cells from 4 mice, 10.7±1.6pF, ApoE−/−Kir2.1L222I:n=6 cells from 4 mice, 12.3 ±2.2pF. *p<0.05, **p<0.01). C. (Left) Representative traces of FIV. (Middle) FIV of the mesenteric arteries from WT and Kir2.1L222I mice with empty (Em) and Cdh5-dnKir2.1 adenoviral vector (WT:n=10 mice, Kir2.1L222I:n=11, *p<0.05). (Right) Comparison of FIV at Δ100 cmH2O (*p<0.05). D. (Left) Representative traces of FIV. (Middle) FIV of the mesenteric arteries from ApoE−/− and Kir2.1L222I/ApoE−/− mice with empty (Em) and Cdh5-dnKir2.1 adenoviral vector (ApoE−/−:n=9, Kir2.1L222I/ApoE−/−:n=11, *p<0.05). (Right) Comparison of FIV at Δ100 cmH2O (*p<0.05).