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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Hypertension. 2021 Nov 17;79(1):126–138. doi: 10.1161/HYPERTENSIONAHA.121.17672

Figure 4. The role of endothelial Kir2.1 in human resistance arterial FIV with elevated LDL cholesterol.

Figure 4.

A. (Left) Cholesterol loading in HAECs after the incubation with 0, 75, 150, and 250 mg/dl LDL for 48 hours. (n=3, *p<0.05) (Right) Current density of Kir2.1 at −100 mV after the incubation with 0, 75, 150, and 250 mg/dl LDL for 48 hours. (n=10–14, *p<0.05) B. (Left) FIV in human subjects with lower than 100 mg/dl LDL cholesterol after 48 hrs incubation with empty viral vector (Em) and Cdh5-dnKir2.1 adenoviral vector (DN) with and without Ba2+ (Ba) (n=11, *p<0.05). (Right) Comparison of FIV at Δ100 cmH2O (n=11, *p<0.05). C. (Left) FIV in human subjects with higher than 100 mg/dl LDL cholesterol under same condition with A (n=9). (Right) Comparison of FIV at Δ100 cmH2O (n=9). D. Correlation of LDL versus Kir-dependent (Left) and Kir-independent (Right) FIV at Δ100 cmH2O (n=20). E. Correlation of FIV and Kir-dependent FIV at Δ100 cmH2O (n=16). (Kir-dependent FIV is defined as Ba2+-(Δ) and Cdh5-dnKir2.1(o)-sensitive FIV. Kir-independent FIV is defined as Ba2+-(Δ) and Cdh5-dnKir2.1(o)-resistant FIV. Correlation Coefficient (r) and *p<0.05)) F. Multiple linear regression models using enter method of factors of interest (LDL, BMI, and age) to predict Kir2.1-dependent maximal FIV in healthy adults (n=20). B=Unstandardized coefficients; BMI=body mass index; LDL=low-density lipoprotein; SE=standard error (*p <0.05).