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. Author manuscript; available in PMC: 2022 Feb 20.
Published in final edited form as: Free Radic Biol Med. 2021 Jan 9;164:315–328. doi: 10.1016/j.freeradbiomed.2020.12.449

Figure 6. Effect of Sod1 deficiency and necroptosis on antioxidant proteins and stress response.

Figure 6.

The relative expression of antioxidant enzymes (A), Lon protease 1 (B), and stress-related proteins (C) in the livers of Sod1KO mice (red bars) and Sod1KO mice treated with Nec-1s (green bars), compared to WT mice, as assessed by targeted quantitative proteomics. Expression of these proteins in WT mice is taken as 100%. The values are normalized to internal standards and housekeeping proteins. (D) Left panel: Immunoblots of liver extracts for 4-HNE. Right panel: Graphical representation of quantified blots normalized to total protein in Coomassie stained gel. Data were obtained from 5 to 6 mice per group and are expressed as the mean ± SEM. (ANOVA, *WT-Veh vs Sod1KO-Veh; #WT-Veh vs Sod1KO-Veh; ^ Sod1KO-Veh vs Sod1KO-Nec-1s; */#/^ P ≤ 0.05).