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Sensitivity of the species-specific p28 PCR assay for E. ewingii with primers EEM2F and EEM1R. A 10-fold dilution series of pMAP template amplified by the p28 PCR assay is shown (lane 1, 3.84 × 107 copies of pMAP, through lane 8, 3.84 copies of pMAP). The expected size (215 bp) is noted. Lanes M show size standards (HaeIII digest of phage φX174 DNA).
