TABLE 4.
PCR, sequencing, and dot blotting results
| Straina | Serotypeb | Genotypec | Ploidyd | MFa genee | MFα genee | STE20af | STE20αf | MFα sequenceg | Dot bloth |
|---|---|---|---|---|---|---|---|---|---|
| CDC B551 | A | VN6 | Haploid | − | + | NDi | ND | 1 | ND |
| IUM 91-4437 | A | VN6 | Haploid | − | + | ND | ND | 1 | ND |
| IUM 94-3443 | A | VN6 | Haploid | − | + | ND | ND | 1 | ND |
| IUM 91-5650 | A | VN6 | Haploid | − | + | ND | ND | 1 | ND |
| IUM 89-6538 | A | VN6 | Haploid | − | + | ND | ND | 1 | ND |
| IUM 87-3588 | A | VN6 | Haploid | − | + | ND | ND | 1 | ND |
| IUM 94-5982 | A | VN6 | Haploid | − | + | ND | ND | 1 | ND |
| IUM 86-0912 | A | VN6 | Haploid | − | + | ND | ND | 1 | ND |
| IUM 90-2798 | A | VN6 | Haploid | − | + | ND | ND | 1 | ND |
| IUM 92-4755 | A | VN6 | Haploid | − | + | ND | ND | 1 | ND |
| IUM 91-6422 | A | VN6 | Haploid | − | + | ND | ND | 1 | ND |
| IUM 95-2499 | A | VN6 | Haploid | − | + | ND | ND | 1 | ND |
| IUM 94-5984 | A | VN6 | Diploid | − | + | n | A | 1 | C6 |
| IUM 95-0552 | A | VN6 | Diploid | − | + | n | A | 1 | C7 |
| IUM 89-6639 | A | VN6 | Diploid | − | +j | n | A | 1 | E2 |
| IUM 88-1358 | AD | VN4 | Diploid | +k | + | D | A | 1 | A4 |
| IUM 89-4797 | A | VN4 | Diploid | +k | + | D | A | 1 | A7 |
| IUM 90-7031 | AD | VN4 | Diploid | +k | + | D | A | 1 | A11 |
| IUM 91-1871 | AD | VN4 | Diploid | +k | + | D | A | 1 | B2 |
| IUM 91-5480 | AD | VN4 | Diploid | +k | + | D | A | 1 | B3 |
| IUM 92-2562 | D | VN4 | Diploid | +k | + | D | A | 1 | B5 |
| IUM 92-4734 | A | VN4 | Diploid | +k | + | D | A | 1 | B8 |
| IUM 93-1666 | D | VN4 | Diploid | +k | + | D | A | 1 | B10 |
| IUM 93-1667 | AD | VN4 | Diploid | + | + | D | A | 1 | B11 |
| RV 53164 | AD | VN4 | Diploid | +k | + | D | A | 1 | C1 |
| IUM 94-3009 | D | VN4 | Diploid | +k | + | D | A | 1 | C3 |
| IUM 94-5754 | AD | VN4 | Diploid | +k | + | D | A | 1 | C4 |
| RV 53565 | AD | VN4 | Diploid | +k | + | D | A | 1 | C5 |
| IUM 95-1584 | AD | VN4 | Diploid | − | + | n | A | 1 | C8 |
| IUM 98-3350 | AD | VN4 | Diploid | +k | + | D | A | 1 | D1 |
| IUM 98-3357 | D | VN4 | Diploid | +k | + | D | A | 1 | D3 |
| RV 29192 | AD | VN4 | Diploid | − | + | n | A | 1 | D7 |
| IUM 98-3351 | AD | VN3 | Diploid | +k | + | D | A | 1 | D2 |
| IUM 98-3359 | D | VN3 | Diploid | +k | + | D | A | 1 | D4 |
| IUM 98-3362 | A | VN3 | Diploid | +k | + | D | A | 1 | D5 |
| RV 52755 | AD | VN3 | Diploid | − | + | n | A | 1 | D9 |
| CBS 132 | AD | VN3 | Diploid | +jk | + | D | A | 1 | E1 |
| IUM 87-2003 | U | VN4 | Diploid | +k | + | A | D | 2 | A3 |
| IUM 89-0130 | D | VN4 | Diploid | +k | + | A | D | 2 | A5 |
| IUM 89-0384 | D | VN4 | Diploid | − | + | n | D | 2 | A6 |
| IUM 88-6669 | U | VN4 | Diploid | +k | + | A | D | 2 | A8 |
| IUM 92-2736 | D | VN4 | Diploid | +k | + | A | D | 2 | B6 |
| RV 28949 | AD | VN4 | Diploid | − | + | n | D | 2 | D6 |
| IUM 90-0243 | D | VN3 | Diploid | + | + | A | D | 2 | A9 |
| IUM 90-5644 | D | VN3 | Diploid | + | + | A | D | 2 | A10 |
| IUM 91-0804 | AD | VN3 | Diploid | + | + | A | D | 2 | A12 |
| IUM 92-1077 | D | VN3 | Diploid | + | + | A | D | 2 | B4 |
| IUM 93-1920 | D | VN3 | Diploid | +k | + | A | D | 2 | B12 |
| IUM 95-4426 | A | VN3 | Diploid | +k | + | A | D | 2 | C9 |
| IUM 95-5227 | AD | VN3 | Diploid | + | + | A | D | 2 | C10 |
| IUM 95-6038 | D | VN3 | Diploid | + | + | A | D | 2 | C11 |
| IUM 96-4769 | D | VN3 | Diploid | − | + | n | D | 2 | C12 |
| IUM 84-3040 | AD | VN4 | Diploid | +k | − | D | n | ND | A1 |
| RV 33370 | AD | VN4 | Diploid | +k | + | D | A | ND | D8 |
| IUM 91-0835 | D | VN3 | Diploid | + | + | A | D | ND | B1 |
| IUM 92-4957 | D | VN3 | Diploid | + | + | A | D | ND | ND |
| IUM 94-1912 | AD | VN3 | Diploid | + | − | A | n | ND | C2 |
| IUM 85-2574 | D | VN4 | Diploid | − | − | ND | ND | ND | ND |
| IUM 86-3543/2 | D | VN1 | Diploid | +k | − | D | n | ND | A2 |
| IUM 92-4686 | D | VN1 | Diploid | +k | + | A | D | 2 | B7 |
| IUM 92-6198 | D | VN1 | Diploid | + | + | A | D | 2 | B9 |
| NIH B4476 | D | VN1 | Haploid | + | − | D | n | 2 | E3 |
| NIH B4500 | D | VN1 | Haploid | − | + | n | D | 2 | E4 |
| IUM 93-1545 | D | VN1 | Haploid | − | + | ND | ND | 2 | ND |
| IUM 89-4478 | D | VN1 | Haploid | − | + | ND | ND | 2 | ND |
| IUM 93-3231 | D | VN1 | Haploid | − | + | ND | ND | 2 | ND |
| IUM 92-6093 | D | VN1 | Haploid | − | + | ND | ND | 2 | ND |
| IUM 88-3921 | D | VN1 | Haploid | − | + | ND | ND | 2 | ND |
| IUM 89-0469 | D | VN1 | Haploid | − | + | ND | ND | 2 | ND |
| IUM 90-2877 | D | VN1 | Haploid | − | + | ND | ND | 2 | ND |
| IUM 91-6367 | D | VN1 | Haploid | − | + | ND | ND | 2 | ND |
| IUM 93-0323 | D | VN1 | Haploid | − | + | ND | ND | 2 | ND |
| IUM 94-0047 | D | VN1 | Haploid | − | + | ND | ND | 2 | ND |
| C. albicans SC5314 | − | − | ND | ND | ND | E5 | |||
| S. cerevisiae DKY1 | − | − | ND | ND | ND | E6 |
IUM, Istituto di Igiene e Medicina Preventiva, Università degli Studi di Milano, Milan, Italy; CBS, Centraalbureau voor Schimmelcultures, Baarn, The Netherlands; NIH, National Institutes of Health, Bethesda, Md.; RV, Raymond Vanbreuseghem, Institut de Médicine Tropical, Anvers, Belgium; CDC Centers for Disease Control and Prevention, Atlanta, Ga.
Determined by the Crypto Check slide agglutination test. U, untypeable.
Determined by PCR fingeprinting using the (GACA)4 primer.
Determined by flow cytometry.
+, gene amplified by PCR and hybridized by dot blotting; −, gene not amplified by PCR and not hybridized by dot blotting.
A, amplification of serotype A allele; D, amplification of serotype D allele; n, not amplified.
1, sequence associated with serotype A, genotype VN6; 2, sequence associated with serotype D, genotype VN1.
Coordinates of the strains tested by dot blotting and reported in Fig. 3.
ND, not done.
Gene amplified by PCR but not detected by dot blotting. Repeat hybridizations confirmed that the classifications by PCR were correct.
Strain in which the MFa gene was sequenced.