FIG. 3.

Infection of PBMCs with C191/VSV-G retroviruses. (A and B) After activation with PHA and rIL-2, the fresh PBMCs were immediately infected with C191/VSV-G retroviruses at an MOI of 80 (A) or stored in liquid nitrogen for a week and then thawed and infected (B). Expression of core antigen was detected by intracellular staining and analyzed with a FACScan. The black peaks show the PBMCs infected with C191/VSV-G retroviruses; the white peaks show the cells infected with S2/VSV-G retroviruses. (C) No changes of HLA type I molecules on PBMCs after retroviral infection. The levels of surface HLA type I molecules on PBMCs with or without retroviral infection were examined with mouse anti-human HLA-A, -B, and -C antibodies. The black peak shows the PBMCs infected with C191/VSV-G retroviruses, the gray peak shows the cells without infection, and the white peak shows the cells stained with the isotype control antibody.