FIG 3.

M. abscessus CFU counts in the lungs of mice. SCID mice were intravenously infected with M. abscessus. One day later, once-daily treatment with either amikacin or different doses of apramycin for 8 consecutive days was started. Mice were euthanized 1 day after receiving the last antibiotic dose. Lungs were homogenized, and extracts were plated on 7H11 agar. A group of three mice was used to calculate the bacterial load at the start of treatment (SOT). One control group (n = 6) did not receive antibiotics (vehicle). Statistical analysis was performed using GraphPad Prism version 5 (GraphPad Software). P values were calculated using one-way ANOVA, and Tukey’s multiple-comparison test. n.s., not significant; **, P < 0.01; ***, P < 0.001.