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BfmR directly interacts with promoter regions of several stress-related genes. Electrophoretic mobility shift assays completed with the indicated promoter DNA and increasing concentrations of untreated BfmR and BfmR treated with the phosphomimic BeF3− (BfmR-BeF3−). The negative-control promoter fragment was an internal fragment from gene I5593_11150 of strain ATCC 17961. Results are representative of at least two independent experiments.
