FIG 3.

BfmR directly activates stress-related transcripts in E. coli. E. coli strain NovaBlue(DE3) carrying the indicated promoters on lacZ-transcriptional fusion plasmids and either the empty expression vector (pET-), pET-BfmR, pET-BfmRS, or pET-BfmR D58A was grown in LB at 37°C, and protein expression was induced as described in Materials and Methods. β-Galactosidase activity was assayed and is presented as the fold change; data are means and SD of results from at least three independent experiments.