FIG 4.

BfmS dephosphorylates BfmR. (A and C) Phos-tag gel and Western blot analysis of the BfmR phosphorylation state in E. coli NovaBlue(DE3) carrying either pET-BfmR or pET-BfmRS (A) or in the wild-type A. baumannii strain ATCC 17961 and the ΔbfmS mutant strain (C). (B and D) Quantification of phosphorylated BfmR as a percentage of total BfmR protein in E. coli strains from panel A (B) or in A. baumannii strains from panel C (D). Data are means and SD from at least three independent experiments.