TABLE 1.
Differentially regulated genes in the ΔbfmR mutant strain versus the wild-type strain ATCC 17961 in key functional groupsa
| Category and locus tag | Gene | Log2 fold change | P value | Predicted function |
|---|---|---|---|---|
| Stress response—osmotic stress | ||||
| I5593_15090 | otsB | −8.05 | 1.17E−106 | Trehalose-6-phosphate phosphatase |
| I5593_15095 | otsA | −7.48 | 1.04E−136 | Trehalose-6-phosphate synthase |
| I5593_15260 | mscS | −5.24 | 2.03E−95 | Small-conductance mechanosensitive channel |
| I5593_07035 | mscM | −4.77 | 1.22E−70 | Mechanosensitive ion channel |
| I5593_06310 | proP | −3.20 | 3.54E−17 | Glycine betaine/l-proline transporter |
| Stress response—oxidative stress | ||||
| I5593_02915 | −6.13 | 6.49E−112 | Alkyl hydroperoxide reductase subunit C-like protein | |
| I5593_16290 | acnA | −4.89 | 1.09E−71 | Aconitate hydratase |
| I5593_01690 | sodC | −4.64 | 2.42E−64 | Superoxide dismutase |
| I5593_11145 | katE | −4.61 | 1.83E−74 | Catalase HPII |
| Stress response—misfolded protein response | ||||
| I5593_19145 | grpE | −3.72 | 5.59E−24 | Nucleotide exchange factor |
| I5593_19150 | dnaK | −3.60 | 7.06E−23 | Molecular chaperone |
| I5593_03940 | groS | −3.35 | 3.34E−24 | Cochaperone |
| I5593_03945 | groL | −3.29 | 4.62E−23 | Chaperonin |
| I5593_17515 | htpG | −3.22 | 2.08E−41 | Molecular chaperone |
| I5593_00180 | dnaJ | −2.80 | 4.55E−21 | Molecular chaperone |
| I5593_13790 | lon | −2.65 | 1.35E−18 | Endopeptidase La |
| I5593_08315 | clpS | −2.32 | 9.07E−18 | ATP-dependent Clp protease adapter |
| I5593_08320 | clpA | −2.23 | 4.50E−14 | ATP-dependent Clp protease ATP-binding subunit |
| I5593_17185 | −2.28 | 3.89E−24 | DnaJ domain-containing protein | |
| I5593_08020 | −2.17 | 1.48E−12 | Similar to ribosome-associated heat shock protein 15 | |
| Stress response—miscellaneous | ||||
| I5593_11130 | absA | −11.20 | 2.9E−201 | A. baumannii stress-related protein A |
| I5593_11155 | cinA 1 | −5.49 | 7.64E−78 | Damage-inducible protein |
| Csu pili/fimbriae | ||||
| I5593_06275 | csuAB | −4.13 | 4.2E−54 | Csu fimbrial major subunit |
| I5593_06280 | csuA | −3.59 | 4.00E−06 | Csu fimbrial biogenesis protein |
| I5593_06290 | csuC | −1.29 | 0.006209 | Csu fimbrial biogenesis chaperone |
| Capsule/polysaccharide biosynthesis | ||||
| I5593_18825 | pgm | −2.37 | 3.62E−18 | Phosphomannomutase/phosphoglucomutase |
| I5593_18835 | gpi | −2.20 | 4.49E−24 | Glucose-6-phosphate isomerase |
| I5593_18840 | ugd | −2.77 | 8.43E−34 | UDP-glucose 6-dehydrogenase |
| I5593_18910 | wza | −1.31 | 1.04E−06 | Polysaccharide biosynthesis/export family protein |
| I5593_18915 | wzb | −1.28 | 5.12E−05 | Low-mol-wt phosphotyrosine protein phosphatase |
| Siderophore biosynthesis and transport | ||||
| I5593_05095 | bauB | 5.81 | 2.70E−28 | Siderophore-binding periplasmic lipoprotein |
| I5593_05085 | bauC | 5.70 | 1.68E−19 | Ferric acinetobactin ABC transporter permease subunit |
| I5593_05080 | bauD | 5.50 | 1.44E−26 | Ferric acinetobactin ABC transporter permease subunit |
| I5593_05090 | bauE | 5.03 | 5.55E−19 | Ferric acinetobactin ABC transporter ATP-binding protein |
| I5593_05075 | basB | 4.81 | 7.81E−57 | Acinetobactin nonribosomal peptide synthetase subunit |
| I5593_05120 | basF | 4.44 | 1.13E−41 | Acinetobactin biosynthesis bifunctional isochorismatase/aryl carrier protein |
| I5593_05115 | basE | 4.14 | 1.27E−52 | (2,3-Dihydroxybenzoyl)adenylate synthase |
| I5593_05125 | basG | 3.67 | 1.08E−23 | Acinetobactin biosynthesis histidine decarboxylase |
| I5593_05110 | basD | 3.26 | 1.83E−29 | Acinetobactin nonribosomal peptide synthetase subunit |
| I5593_05100 | bauA | 3.02 | 1.12E−24 | TonB-dependent ferric acinetobactin receptor |
| I5593_05065 | bauF | 2.66 | 1.14E−26 | Acinetobactin utilization protein |
| I5593_14045 | 2.65 | 8.72E−32 | TonB-dependent siderophore receptor | |
| I5593_05130 | barA | 2.34 | 1.13E−10 | Acinetobactin export ABC transporter permease/ATP-binding subunit |
| I5593_05070 | basA | 2.23 | 9.64E−12 | Acinetobactin nonribosomal peptide synthetase subunit |
| I5593_05135 | barB | 2.15 | 1.22E−09 | Acinetobactin export ABC transporter permease/ATP-binding subunit |
| Type IV pili | ||||
| I5593_01560 | fimT | 8.76 | 2.74E−58 | GspH/FimT family pseudopilin |
| I5593_01565 | pilV | 6.57 | 2.05E−46 | Type IV pilus modification protein |
| I5593_01440 | pilN | 5.61 | 2.59E−34 | PilN domain-containing protein |
| I5593_01445 | pilO | 5.28 | 3.75E−33 | Type 4a pilus biogenesis protein |
| I5593_01570 | pilW | 4.22 | 7.06E−30 | PilW family protein |
| I5593_01450 | pilP | 4.19 | 9.38E−23 | Pilus assembly protein |
| I5593_01575 | pilX | 3.92 | 2.26E−31 | Pilus assembly protein |
| I5593_01435 | pilM | 3.45 | 3.73E−42 | Pilus assembly protein |
| I5593_01585 | 2.76 | 6.15E−17 | Prepilin-type N-terminal cleavage/methylation domain-containing protein | |
| I5593_01580 | pilY | 2.67 | 6.01E−25 | VWA domain-containing protein |
| I5593_17355 | pilB | 2.56 | 1.04E−20 | Type IV-A pilus assembly ATPase |
| I5593_14635 | 2.02 | 8.34E−09 | Type IV pilus twitching motility protein | |
a
The complete set of differentially regulated genes is available in Table S1.