TABLE 3.
Primers used in this study
| Purpose and primer | Sequence (5′→3′)a |
|---|---|
| Generation of mutant strains | |
| bfmS del 1 | AAAAACTGCAGGGTTGTCATGTATCAGTTTG |
| bfmS del 4 | AAAAACTGCAGACAGTCAGTCCGCCTACT |
| bfmS INV 1 | [Phos]TTGTCGAGCGACTCCTTCACT |
| bfmS INV 2 | [Phos] GCATCTGGCGGTTATGGTTTG |
| bfmRS entire 2 F | AAAAAACTGCAGGCGCACTCCATTCTGAATTAA |
| bfmRS entire 3 R | AAAAAACTGCAGATTGCTTGAACATCAATACCTT |
| INV bfmR F | [Phos] ATCATTGCCCCTATAAATCTCATTC |
| INV bfmS R | [Phos] GGTGCTTTTTTATTGGTTTATTTATAATTG |
| bfmR asp ala 1 | TTGGCTGTCATGTTGCCGGGTGCA |
| bfmR asp 2 | GACCACAAGATCCGGTTGCTCACT |
| Cloning transcriptional reporter plasmids | |
| PabsA gfpF | AAAAAAACTGCAGTGTGTATGAATAGGTTGCATCATTC |
| PabsA gfpR2 | AAAAAAAATCTAGACAGGATCCATACTTGCAAATCC |
| cinA_lacZF | AAAAACTGCAGTTTTCACTACAACAGGCATTTTC |
| cinA gfpR | AAAAAAAATCTAGACATTTTAAATCTCCCTACAATTG |
| katE_lacZF | AAAAACTGCAGCATTTTAAATCTCCCTACAATTG |
| PkatE gfpR2 | AAAAAAAATCTAGATTCACTACAACAGGCATTTTC |
| otsBA gfpF | AATTAATTGGTACCAACCTAATTTTAACGATTGCAAAG |
| otsBA gfpR | AAAAAAAATCTAGATCTCCCATCAGATATTATACTTTG |
| proP_lacZF | AAAAACTGCAGGATTATTTAGAGTCAATCCCTG |
| proP gfpR | AAAAAAAATCTAGAGCGCATATCTAGCCTAAGAATTG |
| lacZ-F | AAAAAAAATCTAGAAAGTATAGGAACTTCAGA |
| lacZ-R | GCCGATTCATTAATGCAGC |
| cinA_lacZR | AAAAAAAGCTTCATTTTAAATCTCCCTACAATTG |
| otsBA_lacZF2 | AAAAAAAGCTTAACCTAATTTTAACGATTGCAAAG |
| otsBA_lacZR | AAAAAAAGCTTTCTCCCATCAGATATTATACTTTG |
| proP_lacZR | AAAAAAAGCTTGCGCATATCTAGCCTAAGAATTG |
| PabsA gfpR | AAAAAAAGGTACCTTCAGGATCCATACTTGCAAATCC |
| PkatE gfpR | AAAAAAAAGGTACCTTCACTACAACAGGCATTTTC |
| Cloning expression plasmids | |
| bfmR exR | TTTTTTTTACATGTCGCAAGAAGAAAAGTTACCAAAGATTCTG |
| bfmR exF | TTTTTTTTGGATCCGGAAGTTTAATCAGATTTTACAATCCATTGG |
| BfmSexp_R2 | AAAAAGAATTCCAATAAAAAAGCACCTTATGCAGGTG |
| EMSA analysis | |
| cinA_outF2 | AAAAAAAGCTTGATGATAATACTCGTCTTC |
| bfmR_negR | TCTTGCTAAAGAGATAGATAAACTAGAG |
| absA_lacZF | AAAAAGAATTCTGTGTATGAATAGGTTGCATCATTC |
| absA_lacZR | AAAAAAAGCTTTTCAGGATCCATACTTGCAAATCC |
| cinA_proF | AAGCTAGAAACATCAGGATCAAGG |
| cinA_proR | AAGAGAAACGATAAGCAAGGTAGC |
| otsBA_proF | AACCTAATTTTAACGATTGCAAAG |
| otsBA_proR | TCTCCCATCAGATATTATACTTTG |
| proP_inF | GCGCATATCTAGCCTAAGAATTGAATC |
| proP_proR | GATTATTTAGAGTCAATCCCTGAAGC |
a
Underlining indicates restriction sites.