Figure 5.

Effects of CSF on fecal microbiota. (A) PCoA plot generated by using microbiota OTU metrics based on the Bray‐Curtis similarity for the NS, CSF, NS+HFD, CSF+HFD groups (n = 9/group). (B) The hierarchical cluster based on the Bray‐Curtis similarity of the samples from the NS, CSF, NS+HFD, CSF+HFD groups. The bar plot shows the abundance of each phylum in each sample. (C) The 5 most abundant phyla in the NS, CSF, NS+HFD, CSF+HFD groups. Data are expressed as mean ± SEM. Group differences were assessed by using the Mann‐Whitney U test. ^*P < 0.05 when comparing NS vs CSF, or NS+HFD vs CSF+HFD, ^#P < 0.05 when comparing NS vs NS+HFD. (D) Fold changes of the annotated microbes at the genus level. The data of log₂FC are expressed as mean. Group differences were assessed by using the Mann‐Whitney U test. ^*P < 0.05.