Figure 3.

Serologic detection of MDI-specific IgE and IgG post-mortem. Serum samples obtained at autopsy were tested for the presence of IgE with specificity for MDI by ELISA (A). Study subject IgE binding to MDI (albumin conjugates) is significantly elevated compared to other diisocyanates and results obtained with control sera pooled from (non-diisocyanate asthmatic) healthy adult male subjects. Western blots of the study subject’s serum (B) demonstrate the presence of IgG with specificity for MDI (albumin conjugates) vs. other diisocyanates commonly used commercially, as labeled. Note the triple banding pattern reflective of IgG binding to monomeric, dimerized and trimerized MDI-albumin conjugates, as described in prior studies.²⁵