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. 2022 Feb 15;17(2):e0263829. doi: 10.1371/journal.pone.0263829

Fig 1. HEK-DP engineering.

Fig 1

(a) Schematic of chromosome 19 400 kb pair deletion with (b) example of sequencing at the junction site between DNAJB1 and PRKACA. (c) Comparison of DNA sequence at junction site compared to expected sequence of 12 HEK-DP clones. (d) PKA-Cα immunoblot analysis demonstrated DP fusion protein expression by a 46kD band and wild-type PKA-Cα by a 41kD band. Patient FLC tumor sample (T) and normal liver tissue (N) were used as positive and negative controls, respectively. All colonies (A1-H6) demonstrate expression of DP protein with some clones demonstrating a significantly lower level of wild-type PKA-Cα protein expression (A3, A11, C6, C11). Full-length blots are presented in S1 Fig.