Fig 3. The protein kinases Ire1, Mec1, and Vps15 are required for growth under iron-limiting conditions.

(A) The wild-type strain SC5314, heterozygous and homozygous ire1Δ, mec1Δ, and vps15Δ mutants, and complemented strains were grown in minimal medium with (+ FeCl₃) or without iron (+ BPS). Cultures were photographed after 4 days of growth at 30°C. Both independently generated series of strains are shown. (B) The optical densities of the cultures were determined; shown are the means and standard deviations of three independent cultures of each strain. Data for both independently generated series of strains were combined for statistical analysis. Significant differences from the wild type are indicated by stars (***, p < 0.001). (C) YPD overnight cultures of the strains were serially 10-fold diluted, spotted on YPD agar plates without or with BPS, and grown for 4 days at 30°C.