Skip to main content
. 2022 Feb 2;18(2):e1010283. doi: 10.1371/journal.ppat.1010283

Fig 5. Upregulation of Sef1 and Sef1 target genes by iron depletion does not depend on Ire1, Mec1, and Vps15.

Fig 5

(A) Overnight cultures of the wild-type strain SC5314 and ire1Δ, mec1Δ, and vps15Δ mutants were inoculated in fresh YPD medium without or with BPS and grown for 5 h at 30°C. Expression of the indicated iron-regulated genes was analyzed by Northern hybridization with gene-specific probes; ACT1 mRNA levels served as controls. Each set of experiments was performed separately. (B) The wild-type strain SC5314 and ire1Δ, mec1Δ, and vps15Δ mutants carrying a 3xHA-tagged SEF1 allele were grown as described above and Sef1-HA was detected by Western blotting with an HA-specific antibody. The untagged wild-type strain served as negative control. Both series of mutants are shown in (A) and (B).