Fig 7. Ftr1 is retained in the endoplasmic reticulum in ire1Δ mutants.

Overnight cultures of the wild-type strain SC5314 and ire1Δ mutants carrying a GFP-tagged FTR1 allele were inoculated in fresh YPD medium with BPS, grown for 4 h at 30°C, and stained with ER detection reagent (A) or FM4-64 (B), as described in Materials and methods. Cells were imaged by DIC and fluorescence microscopy using appropriate filters for green and red fluorescence. Shown are DIC images of the cells (top left panels), Ftr1-GFP (top right panels), ER or vacuole staining (bottom left panels), and merged images of Ftr1-GFP and ER/vacuole staining (bottom right panels). The two independently generated series of strains behaved identically and only one of them is shown.