Figure 4. Splice events after transfection of HEK293T cells.

The wildtype SARS-CoV-2 Spike gene – cloned into the three splice trap constructs – was stably transfected in HEK293T cells, RNA was isolated and investigated by RT-PCR experiments for splicing events. Appropriate PCR bands were cut out from the gel and either directly sequenced or cloned for sequencing. The major splice events discovered are displayed.