Figure 5. Splice events after Ad5.S transduction of HeLa cells.

HeLa or HepG2 cells were transduced with the adenoviral vectors Ad5.S and RNA was isolated 48 hr post transduction. Three universal primers binding at three different locations within the Spike genes and one universal reverse primer binding to the adenoviral pIX that is located directly adjacent to the Spike gene sequence were used to investigate splicing events. The resulting DNA fragments are shown in the agarose gel on the left. Appropriate PCR bands were excised from the gel and either directly sequenced or cloned for sequencing. The predominately detected splice events are displayed on the right.