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. 2022 Jan 24;11:e69094. doi: 10.7554/eLife.69094

Figure 7. Long-term 2P imaging of odor-evoked responses of the mushroom body γ-lobes.

Figure 7.

(A) Stimulus timeline for long-term odor imaging. GCaMP6s fluorescence signal is captured from Kenyon cells axons innervating mushroom body γ-lobes using the semi compressed preparation. (B–E) Quantification of the normalized signal (ΔF/F0) over time in each γ-lobe compartment. Light orange bar indicates when the odor stimulus is present. Each colored line indicates the average response of a fly over multiple trials in a given hour. The average response of three flies is shown. Each compartment’s response is labeled with a different color. (F) Quantification of the peak amplitude across different time points and lobes (dF/F0) (Two-way repeated measures ANOVA. Data are presented as mean ± SEM, ns, not significant; n = 3 flies, 3 trials per time point). Average laser power at 920 nm is <10 mW. Images were captured at 256 × 128 pixels/frame and 17 Hz frame rate. 2P data were averaged to 5 Hz effective sampling rate for plotting.

Figure 7—source data 1. Source data for plots Figure 7B-E (t=0hr).
elife-69094-fig7-data1.xlsx (977.6KB, xlsx)
Figure 7—source data 2. Source data for plot Figure 7B-E (t=4hr).
elife-69094-fig7-data2.xlsx (977.7KB, xlsx)
Figure 7—source data 3. Source data for plots Figure 7B-E (t=8hr).
elife-69094-fig7-data3.xlsx (977.7KB, xlsx)
Figure 7—source data 4. Source data for plots Figure 7B-E (t=12hr).
elife-69094-fig7-data4.xlsx (976.1KB, xlsx)
Figure 7—source data 5. Source data for plot Figure 7F.