Table 1. Surface markers for flow cytometry.
Hematopoietic cell populations identified by flow cytometry were characterized using the listed surface markers. Lineage (Lin) markers include Gr1, CD11b, B220, CD4, CD8, and Ter119.
| Population | Markers |
|---|---|
| HSC | Lin- ckit+ CD150+ CD48- CD34- Flk2- |
| CD41+ HSCs | Lin- ckit+ CD150+ CD48- CD34- Flk2- CD41+ |
| Donor HSPCs | Lin- ckit+ Sca1+ |
| HSPCs | Lin- ckit+ |
| MPP1 | Lin- ckit+ CD150+ CD48 CD34+ Flk2- |
| MPP2 | Lin- ckit+ CD150+ CD48+ Flk2- |
| MPP3 | Lin- ckit+ CD150 CD48+ CD34+ Flk2- |
| MPP4 | Lin- ckit+ CD150 CD48+ CD34+ Flk2+ |
| GMP | Lin- ckit+ CD41- CD150- CD16/32+ |
| MkP | Lin- ckit+ CD150+ CD41+ |
| Myeloid cells | Gr1+ Mac1+ B220- CD4- CD8- |
| B cells | Gr1- Mac1- B220+ CD4- CD8- |
| T cells | Gr1- Mac1- B220- CD4+ CD8 or Gr1- Mac1- B220- CD4- CD8+ |
| Granulocytes | Gr1+ Mac1+ B220- CD4- CD8- SSC-mid F4/80- |
| Eosinophils | Gr1- Mac1+ B220- CD4- CD8- SSCHi |
| Macrophages | Gr1+ Mac1+ B220- CD4- CD8- SSClow F4/80+ |
| Monocytes | Mac1+ B220- CD4- CD8- SSClow |
| PMN-MDSC | CD11b + Ly6G + Ly6ClowCD244+ |
| M-MDSC | CD11b + Ly6G- Ly6Chi |