| Strain, strain background (Mus musculus; male) |
BKS(D)-Leprdb/JOrlRj, Leprdb/db diabetic mice |
Janvier Labs |
RRID:MGI:6293869
|
|
| Strain, strain background (Mus musculus; male) |
C57BL/6JRj Mouse |
Janvier Labs |
RRID:MGI:5751862
|
|
| Strain, strain background (Mus musculus; male) |
Egln1+/- and wild-type mice |
Own colony |
PMID:19217150Mazzone et al., 2009
|
|
| Cell line (Mus musculus) |
mIMCD-3 cell line |
ATCC |
Cat#:CRL-2123;RRID:CVCL_0429
|
|
| Transfected construct (M. musculus) |
siRNA to mouse VHL
|
Qiagen |
Gene Solution siRNA (Cat#: 1027416) |
Target sequence: TCCGAGATTGATCTACACATA |
| Transfected construct (M. musculus) |
AllStars Negative Control siRNA |
Qiagen |
Cat#: 1027280 |
|
| Antibody |
anti-HIF-1alpha (Rabbit polyclonal) |
GeneTex |
Cat#: GTX127309; RRID:AB_2616089
|
ICC(1:200)IHC(1:100) |
| Antibody |
anti-KIM-1 (Rabbit polyclonal) |
Novus Biologicals |
Cat#:NBP1-76701;RRID:AB_11037459
|
IHC(1:50)WB(1:500) |
| Antibody |
Goat anti-Rabbit Secondary Antibody, Alexa Fluor 594 |
Thermo Fisher Scientific |
Cat#:A-11037;RRID:AB_2534095
|
ICC (1:500)IHC (1:500) |
| Antibody |
Goat anti-Rabbit Secondary Antibody, Alexa Fluor 488 |
Thermo Fisher Scientific |
Cat#:A-11008;RRID:AB_143165
|
IHC (1:500)WB (1:500) |
| Antibody |
anti-HIF-1alpha (Rabbit polyclonal) |
Novus Biologicals |
Cat#:NB100-479;RRID:AB_10000633
|
WB: 1:500 |
| Antibody |
anti-Histone H3 (Rabbit polyclonal) |
Abcam |
Cat#: ab1791;RRID:AB_302613
|
WB: 1:5,000 |
| Antibody |
anti-α-tubulin (mouse monoclonal) |
Abnova |
Cat#:MAB11106; RRID:AB_2888691
|
WB:1:1,000 |
| Antibody |
IRDye 800 goat anti-rabbit Secondary Antibody |
LI_COR Biosciences |
Cat#:925–32211; RRID:AB_2651127
|
WB:1:20,000 |
| Antibody |
IRDye 680 goat anti-mouse Secondary Antibody |
LI_COR Biosciences |
Cat#:925–68070; RRID:AB_2651128
|
WB:1:20,000 |
| Recombinant DNA reagent |
pCMV3-FLAG-PDK1 |
Sino Biological Inc |
Cat#: HG12312-NF |
Plasmid encoding FLAG-tagged human PDK1 |
| Recombinant DNA reagent |
pCMV3-GFP-FLAG-PDK1 |
This paper |
|
Plasmid encoding GFP-fused FLAG-tagged human PDK1 |
| Sequence-based reagent |
Mouse PDK1_F |
This paper |
PCR primers |
AGTCCGTTGTCCTTATGAG |
| Sequence-based reagent |
Mouse PDK1_R |
This paper |
PCR primers |
CAGAACATCCTTGCCCAG |
| Sequence-based reagent |
Mouse BNIP3_F |
This paper |
PCR primers |
AACAGCACTCTGTCTGAGG |
| Sequence-based reagent |
Mouse BNIP3_R |
This paper |
PCR primers |
CCGACTTGACCAATCCCA |
| Sequence-based reagent |
Mouse PGK1_F |
This paper |
PCR primers |
AGTCCGTTGTCCTTATGAG |
| Sequence-based reagent |
Mouse PGK1_R |
This paper |
PCR primers |
CAGAACATCCTTGCCCAG |
| Sequence-based reagent |
MouseSDF-1alpha_F |
This paper |
PCR primers |
GAGAGCCACATCGCCAGAG |
| Sequence-based reagent |
MouseSDF-1alpha_R |
This paper |
PCR primers |
TTTCGGGTCAATGCACACTTG |
| Sequence-based reagent |
MouseEgln1_F |
This paper |
PCR primers |
GGGCAACTACAGGATAAACGG |
| Sequence-based reagent |
Mouse Egln1_R |
This paper |
PCR primers |
CTCCACTTACCTTGGCGT |
| Sequence-based reagent |
Mouse GLUT3_F |
This paper |
PCR primers |
TCATCTCCATTGTCCTCCAG |
| Sequence-based reagent |
Mouse GLUT3_R |
This paper |
PCR primers |
CCAGGAACAGAGAAACTACAG |
| Sequence-based reagent |
MouseACTB_F |
This paper |
PCR primers |
AAGATCAAGATCATTGCTCCTC |
| Sequence-based reagent |
MouseACTB_R |
This paper |
PCR primers |
GGACTCATCGTACTCCTG |
| Sequence-based reagent |
MouseHMBS_F |
This paper |
PCR primers |
CCTGTTCAGCAAGAAGATGGTC |
| Sequence-based reagent |
MouseHMBS_R |
This paper |
PCR primers |
AGAAGTAGGCAGTGGAGTGG |
| Sequence-based reagent |
MouseVHL_F |
This paper |
PCR primers |
CATCACATTGCCAGTGTATACCC |
| Sequence-based reagent |
MouseVHL_R |
This paper |
PCR primers |
GCTGTATGTCCTTCCGCAC |
| Commercial assay or kit |
MycoAlert PLUS mycoplasma detection kit |
LONZA |
Cat#:LT07-218 |
|
| Commercial assay or kit |
Dual-Luciferase Reporter Assay System |
Promega |
Cat#: E1960 |
|
| Commercial assay or kit |
Annexin V-FITC / 7-AAD kit |
Beckman Coulter |
Cat#: IM3614 |
|
| Commercial assay or kit |
Caspase-Glo 3/7 assay kit |
Promega |
Cat#: G8091 |
|
| Commercial assay or kit |
Quant-iT dsDNA High-Sensitivity Assay Kit |
Thermo Fisher Scientific |
Cat#: Q33120
|
|
| Commercial assay or kit |
Lipofectamine RNAiMAX Transfection Reagent |
Thermo Fisher Scientific |
Cat#: 13778075 |
|
| Commercial assay or kit |
MitoSOX Red Mitochondrial Superoxide Indicator, for live-cell imaging |
Thermo Fisher Scientific |
Cat#:M36008
|
|
| Commercial assay or kit |
ProLong Gold Antifade Mountant with DAPI |
Thermo Fisher Scientific |
Cat#:P36935
|
|
| Commercial assay or kit |
DAPI |
Thermo Fisher Scientific |
Cat#:D1306 |
|
| Commercial assay or kit |
Hypoxyprobe–1 Omni Kit |
Hypoxyprobe, Inc |
Cat#:HP1-XXX |
|
| Commercial assay or kit |
Tyramide Superboost kit |
Thermo Fisher Scientific |
Cat#:B40943
|
|
| Commercial assay or kit |
OxiSelectTM HNE Adduct Competitive ELISA kit |
Cell Biolabs |
STA838 |
|
| Commercial assay or kit |
DC Protein Assay |
BIO-RAD |
Cat#:5000111 |
|
| Commercial assay or kit |
miRNeasy Mini kit |
Qiagen |
Cat#:217,004 |
|
| Commercial assay or kit |
High-Capacity cDNA Reverse Transcription Kit |
Thermo Fisher Scientific |
Cat#:4368814 |
|
| Commercial assay or kit |
SYBR Green Master Mix |
Thermo Fisher Scientific |
Cat#:4367659 |
|
| Commercial assay or kit |
Bradford Protein Assay |
BIO-RAD |
Cat#:5000001 |
|
| Commercial assay or kit |
In Situ Cell Death Detection Kit |
Roche |
Cat#:11684817910RRID:AB_2861314
|
|
| Commercial assay or kit |
DCA Microalbumin/Creatinine Urine Test |
Siemens Healthcare GmbH |
Cat#:01443699 |
|
| Chemical compound, drug |
CPH (1-hydroxy-3-carboxy-pyrrolidine) |
Noxygen Science Transfer & Diagnostics GmbH |
Cat#:NOX-01.1–50 mg |
|
| Chemical compound, drug |
EPR-grade Krebs HEPES buffer |
Noxygen Science Transfer & Diagnostics GmbH |
Cat#:NOX-7.6.1–500 ml |
|
| Chemical compound, drug |
Deferoxamine |
Noxygen Science Transfer & Diagnostics GmbH |
Cat#:NOX-09.1–100 mg |
|
| Chemical compound, drug |
DETC (diethyldithiocarbamate) |
Noxygen Science Transfer & Diagnostics GmbH |
Cat#:NOX-10.1–1 g |
|
| Chemical compound, drug |
DMOG (Dimethyloxalylglycine) |
Frontier Specialty Chemicals |
Cat#:D1070 |
|
| Chemical compound, drug |
cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail |
Roche |
Cat#: 11836170001 |
|
| Chemical compound, drug |
Formaldehyde solution |
Sigma |
Cat#: F8775 |
|
| Chemical compound, drug |
Streptozotocin |
Sigma |
Cat#: S0130 |
|
| Chemical compound, drug |
Sudan Black B |
Sigma |
Cat#:199,664 |
|
| Software, algorithm |
FlowJo |
FlowJo |
RRID:SCR_008520
|
|
| Software, algorithm |
Image-Pro Premier v9.2 |
Media Cybernetics |
|
|
| Software, algorithm |
ImageJ |
ImageJ |
RRID:SCR_003070
|
|
| Software, algorithm |
GraphPad Prism |
GraphPad Prism |
RRID:SCR_002798
|
|
| Other |
Dulbecco’s Modified Eagle’s Medium |
Thermo Fisher Scientific |
31885–023 |
|
