| Strain, strain background (C57BL/6 J) |
C57BL/6 J |
Jackson laboratories |
000664 |
|
| Strain, strain background (C57BLKS/J) |
C57BLKS-m Leprdb/db
|
Jackson laboratories |
000662 |
|
| Antibody |
Anti-Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5)(Mouse monoclonal) |
Thermo Fisher Scientific |
Cat# MA1-10401, RRID:AB_11152791
|
For neutrophil depletion (100 µg/mouse) |
| Antibody |
Anti-Mouse (G3A1) mAb IgG1 Isotype Control antibody(Mouse monoclonal) |
Cell Signaling Technologies |
Cat#5415, RRID:AB_10829607
|
For neutrophil depletion (100 µg/mouse) |
| Antibody |
GAPDH antibody (Rabbit polyclonal) |
Proteintech |
Cat# 1094-I-AP, RRID:AB_2895245
|
WB (1:10000) |
| Antibody |
Anti-Ly6G antibody clone RB6-8C5 (Rat monoclonal) |
Abcam |
Cat# ab25377, RRID:AB_470492
|
IHC (1:50) |
| Antibody |
Anti-FPR1 antibody(Rabbit polyclonal) |
NOVUS Biological |
Cat# NB100-56473, RRID:AB_838228
|
WB (1:1000) |
| Antibody |
Anti-FPR2/ FPRL1 antibody(Rabbit polyclonal) |
NOVUS Biologicals |
Cat# NLS1878, RRID:AB_2294156
|
WB (1:1000) |
| Antibody |
Anti-PLC1 antibody(Rabbit polyclonal) |
Cell Signaling Technology |
Cat# cs2822, RRID:AB_2163702
|
WB (1:1000) |
| Antibody |
Anti-CCR1 antibody(Rabbit polyclonal) |
Abnova |
Cat# PAB0176, RRID:AB_1018941
|
WB (1:500) |
| Antibody |
Anti-α-SMA antibody(Rabbit polyclonal) |
Abcam |
Cat# ab5694, RRID:AB_2223021
|
|
| Antibody |
Anti-vimentin antibody(Rabbit monoclonal) |
Abcam |
Cat# ab92547, RRID:AB_10562134
|
|
| Antibody |
Mouse CCR1 Alexa Fluor 488-conjugated Antibody(Rat monoclonal) |
NOVUS Biologicals |
Cat# FAB5986G, RRID:AB_2895246
|
Flow cytometery |
| Antibody |
Alexa Fluor 700 anti-mouse NK-1.1 Antibody(Mouse monoclonal) |
BioLegend |
Cat# 108729, RRID:AB_2074426
|
Flow cytometery |
| Antibody |
Alexa Fluor 700 anti-mouse CD3ε Antibody(Syrian Hamster monoclonal) |
BioLegend |
Cat# 152315, RRID:AB_2632712
|
Flow cytometery |
| Antibody |
Alexa Fluor 700 anti-mouse CD19 Antibody(Rat monoclonal) |
BioLegend |
Cat# 115527, RRID:AB_493734
|
Flow cytometery |
| Antibody |
BV605 Hamster Anti-Mouse CD11c Clone HL3 (RUO)(Hamster monoclonal) |
BD Biosciences |
Cat# 563057, RRID:AB_2737978
|
Flow cytometery |
| Antibody |
F4/80 antibody, Cl:A3-1(Rat monoclonal) |
Bio-Rad |
Cat# MCA497PBT, RRID:AB_1102557
|
Flow cytometery Flow cytometery |
| Antibody |
BV650 Hamster Anti-Mouse CD11c Clone HL3(Hamster monoclonal) |
BD Biosciences |
Cat# 564079, RRID:AB_2725779
|
Flow cytometery |
| Antibody |
BV711 Rat Anti-Mouse CD45 Clone 30-F11(Rat monoclonal) |
BD Biosciences |
Cat# 563709, RRID:AB_2687455
|
Flow cytometery |
| Antibody |
NK1.1 Monoclonal Antibody (PK136), PE, eBioscience(Mouse monoclonal) |
Thermo Fisher Scientific |
Cat# 12-5941-82, RRID:AB_466050
|
Flow cytometery |
| Antibody |
CD19 Monoclonal Antibody (eBio1D3 (1D3)), PE, eBioscience(Rat monoclonal) |
Thermo Fisher Scientific |
Cat# 12-0193-82, RRID:AB_657659
|
Flow cytometery |
| Antibody |
CD3e Monoclonal Antibody (145–2 C11), PE, eBioscience(Hamster monoclonal) |
Thermo Fisher Scientific |
Cat# 12-0031-82, RRID:AB_465496
|
Flow cytometery |
| Antibody |
FPR1 Polyclonal Antibody(abbit polyclonal) |
Thermo Fisher Scientific |
Cat# PA1-41398, RRID:AB_2247097
|
Flow cytometery |
| Antibody |
Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594(Goat polyclonal) |
Thermo Fisher Scientific |
Cat# A-11037, RRID:AB_2534095
|
Flow cytometery |
| Antibody |
Ly6G Monoclonal Antibody (1A8-Ly6g), PE-Cyanine7, eBioscience(Rat monoclonal) |
Thermo Fisher Scientific |
Cat# 25-9668-82, RRID:AB_2811793
|
Flow cytometery |
| Antibody |
PerCP Cy5.5 CD45 antibody(Rat monoclonal) |
BD Biosciences |
Cat# 550994, RRID:AB_394003
|
Flow cytometery |
| Antibody |
APC Gr1, PE CD11b antibody(Rat monoclonal) |
BD Biosciences |
Cat# 553129, RRID:AB_398532
|
Flow cytometery |
| Antibody |
FITC CD69 antibody(Hamster monoclonal) |
BD Biosciences |
Cat# 557392, RRID:AB_396675
|
Flow cytometery |
| Antibody |
PECy7 F4/80 antibody(Rat monoclonal) |
BioLegend |
Cat# 123114, RRID:AB_893478
|
Flow cytometery |
| Commercial assay or kit |
LIVE/DEAD Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation |
ThermoFisher Scientific |
Cat# L34966
|
|
| Sequence-based reagent |
FPR1_F |
Integrated DNA Technologies |
RT-PCR primers |
GAGCCTAGCCAAGAAGGTAATC |
| Sequence-based reagent |
FPR1_R |
Integrated DNA Technologies |
RT-PCR primers |
TCCCTGGTCCAAGTCTACTATT |
| Sequence-based reagent |
FPR2_F |
Integrated DNA Technologies |
RT-PCR primers |
TTGTCTCAATCCGATGCTCTATG |
| Sequence-based reagent |
FPR2_R |
Integrated DNA Technologies |
RT-PCR primers |
TCAGGGCTCTCTCAAGACTATAA |
| Sequence-based reagent |
Plcg1_F |
Integrated DNA Technologies |
RT-PCR primers |
GGTGAGGCCAAATGTGAGATA |
| Sequence-based reagent |
Plcg1_R |
Integrated DNA Technologies |
RT-PCR primers |
GGGCAACCAAGAGGAATGA |
| Sequence-based reagent |
Ccr1_F |
Integrated DNA Technologies |
RT-PCR primers |
GCTATGCAGGGATCATCAGAAT |
| Sequence-based reagent |
Ccr1_R |
Integrated DNA Technologies |
RT-PCR primers |
GGTCCAGAGGAGGAAGAATAGA |
| Sequence-based reagent |
Ccl3_F |
Integrated DNA Technologies |
RT-PCR primers |
TCACTGACCTGGAACTGAATG |
| Sequence-based reagent |
Ccl3_R |
Integrated DNA Technologies |
RT-PCR primers |
CAGCTTATAGGAGATGGAGCTATG |
| Sequence-based reagent |
GAPDH_F |
Integrated DNA Technologies |
RT-PCR primers |
TTGGGTTGTACATCCAAGCA |
| Sequence-based reagent |
GAPDH_R |
Integrated DNA Technologies |
RT-PCR primers |
CAAGAAACAGGGGAGCTGAG |
| Sequence-based reagent |
18 S_F |
Integrated DNA Technologies |
RT-PCR primers |
CACGGACAGGATTGACAGATT |
| Sequence-based reagent |
18 S_R |
Integrated DNA Technologies |
RT-PCR primers |
GCCAGAGTCTCGTTCGTTATC |
| Commercial assay or kit |
Myeloperoxidase (MPO) Mouse ELISA Kit |
Thermo Fisher Scientific |
Cat# EMMPO |
|
| Commercial assay or kit |
IL-1b ELISA kit |
Thermo Fisher Scientific |
Cat# 88-7013-88 |
|
| Commercial assay or kit |
TNF- a ELISA kit |
Thermo Fisher Scientific |
Cat# 88-7324-88 |
|
| Commercial assay or kit |
Cyclic AMP Competitive ELISA Kit |
Cayman chemical |
Cat# 581,001 |
|
| Commercial assay or kit |
EasySep Human Monocytes Enrichment Kit |
STEMCELL Technologies |
Cat# 19,359 |
|
| Commercial assay or kit |
EasySep Mouse monocytes Enrichment Kit |
STEMCELL Technologies |
Cat# 19,861 |
|
| Commercial assay or kit |
SuperScript III First-Strand Synthesis System |
Thermo Fisher |
Cat# 18080051 |
|
| Peptide, recombinant protein |
CCL3 (recombinant mouse CCL3/MIP-1α protein) |
R & D Systems |
Cat# 450-MA |
|
| Peptide, recombinant protein |
N-formyl-Met-Leu-Phe (fMLP) |
Sigma |
Cat# 59880-97-6 |
|
| Peptide, recombinant protein |
Recombinant Human CXCL1/GRO alpha Protein |
R & D Systems |
Cat# 275-GR |
|
| Peptide, recombinant protein |
Recombinant Mouse CXCL1/KC Protein |
R & D Systems |
Cat# 453-KC |
|
| Software, algorithm |
GraphPad |
GraphPad |
https://graphpad.com/scientific-software/prism/
|
|
| Other |
Hematoxylin |
Thermo Fisher Scientific |
Cat# 7,111 L |
|
| Other |
Eosin Y |
Thermo Fisher Scientific |
Cat# 7,211 L |
|
| Other |
Bluing Reagent |
Thermo Fisher Scientific |
Cat# 7,301 L |
|
| Other |
Masson’s Trichrome stain |
Abcam |
Cat# ab150686 |
|
| Other |
EasySep Buffer |
STEMCELL Technologies |
Cat. No. 20,144 |
|
| Other |
SYBR Green PCR Master Mix |
Thermo Fisher |
Cat. No. 4309155 |
|
| Other |
Collagenase D |
Sigma |
Cat# 9001-12-1 |
|
| Other |
Calcein AM |
Thermo Fischer Scientific |
Cat# C1430 |
|
