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. Author manuscript; available in PMC: 2022 Jul 5.
Published in final edited form as: Eur J Med Chem. 2021 Apr 8;219:113435. doi: 10.1016/j.ejmech.2021.113435

Figure 4.

Figure 4.

A. Cell viability assay of 4EGI-1 and i4EG-BiP in two different cell lines (MCF7 and MM1S) as a function of concentration over 24 h, 48 h and 72 h. Error bars represent standard deviation and in all cases n=3. B. A dual luciferase assay for 4EGI-1 and i4EG-BiP shows a greater decrease in cap-dependent translation with increasing compound concentration for i4EG-BiP. C. Western blot of an eIF4E pull down with m7-GDP functionalized agarose beads in the absence and presence of increasing concentrations of i4EG-BiP. With increasing concentrations of i4EG-BiP, the eIF4E/eIF4G ratio increases, while the eIF4E/4E-BP ratio decreases.