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. 2022 Feb 8;49(4):47. doi: 10.3892/ijmm.2022.5102

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Copyright: © Kong et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Experimental validation of PXR as a target gene of mmu-miR-599. (A) Mmu-miR-599 potential binding sites on the 3'untranslated region of PXR. (B) Relative luciferase activity. ^***P<0.001 compared with mimic control group. (C) Expression levels of PXR regulated by mmu-miR-599 mimic. ^***P<0.001 compared with control group; ^###P<0.001 compared with mimic control group. (D) Expression levels of PXR regulated by mmu-miR-599 inhibitor. ^***P<0.001 compared with control group; ^###P<0.001 compared with inhibitor control group. Data are expressed as the mean ± SD (n=6 per group). PXR, pregnane X receptor; miR, microRNA; WT, wild type; MUT, mutant.