Table II.
Primer sequences used for reverse transcription-quantitative PCR analysis.
| Primer name | Product length (bp) | Primer sequence (5'-3') |
|---|---|---|
| mmu-microRNA-599 | 88 | F: TGCTGTCCACAGTGTATTTGAT |
| R: GTCATCCAGGTATGGGTTTG | ||
| PXR | 97 | F: GCATCCTGAGGTTCAACACG |
| R: TGGAAGCCACCATTAGGGT | ||
| NF-κB | 168 | F: GCGAGAGAAGCACAGATACCA |
| R: GGTCAGCCTCATAGTAGCCAT | ||
| TNF-α | 300 | F: GAACTGGCAGAAGAGGCACT |
| R: AGAAGAGGCTGAGACATAGGC | ||
| IL-1β | 99 | F: AGCCCATCCTCTGTGACTCA |
| R: TTTGTTGTTCATCTCGGAGCC | ||
| IL-6 | 131 | F: TACCACTCCCAACAGACCTG |
| R: TCTCATTTCCACGATTTCCCAG | ||
| NLRP3 | 128 | F: GAAGAAGAGTGGATGGGTTTG |
| R: GCGTTCCTGTCCTTGATAGAGT | ||
| STAT3 | 142 | F: TGGCACCTTGGATTGAGAG |
| R: TGCTGATAGAGGACATTGGACT | ||
| β-actin | 144 | F: GACAGGATGCAGAAGGAGATTACTG |
| R: GCTGATCCACATCTGCTGGAA |
PXR, pregnane X receptor; NF-κB, nuclear factor-κB; TNF-α, tumour necrosis factor-α; IL, interleukin; NLRP3, NOD-like receptor family pyrin domain-containing protein 3; STAT 3, signal transducer and activator of transcription 3; F, forward; R, reverse.