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. 2022 Feb 1;12:807398. doi: 10.3389/fmicb.2021.807398

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Copyright © 2022 Gargis, Spicer, Kent, Zhu, Campbell, McAllister, Ewing, Albrecht, Stevens, Sheth, Padilla, Batra, Johnson, Halpin, Rasheed, Elkins, Karlsson and Lutgring.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparative analysis of pAR-0780 with similar optrA-encoding plasmids. pAR-0780 contained a near identical copy (100% query coverage, 99.95% identity) of a 14,349 bp optrA gene cluster flanked on the left- and right-hand sides by IS1216R elements (black) previously described on plasmid p10-2-2 from an E. faecalis isolate (ST59) recovered from a pig in China in 2015. The remaining 51,543 bp of pAR-0780 was nearly identical (100% coverage, 99.88% identity) to a 51,543 region of the 84,468 bp plasmid, pE508, a pheromone-responsive conjugative multiresistance plasmid from E. faecalis strain E508 (ST256), isolated from a swine fecal sample in 2015.