Fig. 3.

2-DG treatment reduces the proliferation efficiency of Vero E6 cells.

A. SRB assay was performed to examine the effect of 2-DG on cell proliferation. The graph shows the relative proliferation efficiency (%) of Vero E6 cells at different indicated concentrations of 2-DG with respect to control. B–C. Bar plots indicate the relative uptake in Annexin V/PI (B) and percent of live vs. dead cell population (C) obtained from trypan blue exclusion assay in the indicated treatment groups. D–E. CFSE proliferation assay was performed, and a differential overlay graph obtained in the indicated treatment groups [1 mM] (D) and [5 mM] (E) shows the proliferation-dependent fluorescence shift in Vero E6 cells. F. The barplot represents the reproductive potential/clonogenicity of 2-DG treated cells at 1 and 5 [mM], with respect to control. All barplots show the mean ± standard deviation (SD) obtained from three independent observations (n = 3) and, P-values were calculated with a two-tailed Student's t-test with respect to uninfected control. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)