Figure 3.

FAM83D was one of the molecules downstream of YTHDF2. (A) Volcano map of proteins in pathways downstream of YTHDF2. (B) Expression of eleven survival-related proteins in pathways downstream of YTHDF2 in lung adenocarcinoma. (C) FAM83D protein expression level after YTHDF2 knockdown, as measured using WB. (D) FAM83D mRNA levels after YTHDF2 knockdown, as measured using qRT–PCR. (E) RIP assay showing that the FAM83D mRNA was enriched by YTHDF2 antibody-bound magnetic beads, and actin was used as a negative control. (F) Bands of three independent RIP assays analysed using DNA agarose gel electrophoresis. (G) Prediction of m6A binding sites in FAM83D mRNA. *Statistical difference (P < 0.05).