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. 2022 Feb 2;12:763341. doi: 10.3389/fonc.2022.763341

Figure 4.

Figure 4

YTHDF2 negatively regulates the FAM83D-TGFβ1-pSMAD2/3 pathway. (A) FAM83D expression in different patient subgroups compared with normal controls in TCGA cohort. (B) Analysis of OS of patients with lung adenocarcinoma from different FAM83D expression groups in TCGA cohort. (C) Wound healing assay using normal and FAM83D knockdown cell lines. (D) Transwell assay using normal and FAM83D knockdown cell lines. (E) Levels of TGFβ1-pSMAD2/3 signalling proteins and EMT pathway components in normal and FAM83D knockdown cell lines were measured using WB, and the relative greyscale values of 3 repetitions are shown. (F) Levels of TGFβ1-pSMAD2/3 signalling proteins in normal and YTHDF2 knockdown cell lines were measured using WB, and the relative greyscale values of 3 repetitions are shown. (G, H) The rescue experiment after YTHDF2 expression was knock down in A549 and 1299 cell lines. NC, normal compare; siRNA, knockdown group; OE-siRNA, overexpression by plasmid after knockdown by siRNA group. *Statistical difference (P < 0.05).