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. 2022 Feb 2;10:672391. doi: 10.3389/fcell.2022.672391

FIGURE 3.

FIGURE 3

LncAABR07025387.1 correlated with ACSL4–LPCAT3–mediated ferroptosis in vitro. (A) LncAABR07025387.1-specific siRNA or overexpression vectors were transfected into H9C2 cells before H/R exposure. LncAABR07025387.1 expression was detected using qRT-PCR. (B) Cell viability was evaluated using CCK-8 assays 24 h after transfection. (C) Iron accumulation was monitored at 24 h in cells with lncAABR07025387.1 overexpression or downregulation, followed by H/R exposure. (D) Level of ROS in myocardial tissues after transfection. (E) Representative blots and analyses of ACSL4 and LPCAT3 in H/R-exposed cells after transfection. (F) Real-time PCR analysis of gene expression in cells with different treatments. (G) Intracellular ROS levels measured using fluorescence assays. Bar = 50 µm. Data represent the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. the H/R + vector or H/R + si-ctrl groups. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. the control group.