FIGURE 5.

ACSL4 is a target gene of miR-205. (A) Predicted binding sites of miR-205 in the ACSL4 transcript. (B) Luciferase activities were determined in HEK293T cells co-transfected with luciferase reporters containing a wild-type or mutant ACSL4 3′-UTR and an miR-205 mimic or inhibitor. Luciferase activity was normalized to Renilla luciferase. **p < 0.01 vs. the control group. (C) Schematic diagram of the RIP assay. (D) Immunoprecipitated mRNA was isolated and the levels of ACSL4 and miR-205 were measured using qRT-PCR. (E) Representative blots and analyses of ACSL4 and LPCAT3 in H/R-exposed cells after miR-205 mimic transfection with or without the ACSL4 overexpression vector. ^** p < 0.01 vs. the untreated group. ^## p < 0.01 vs. the H/R + control group. ^$ p < 0.05 vs. the H/R + miR-205 mimic group.