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. 2021 Nov 8;157(2):127–137. doi: 10.1007/s00418-021-02037-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — FTIR data analysis via random decision forest classification of a tissue section of E. fetida. a Result of the RDF application for class 1 “background” as overlay over the optical image of the section and an exemplary IR spectrum representing this class; this class is represented with blue coloration. b Result of the RDF application for class 2 “body wall” as overlay over the optical image of the section and an exemplary IR spectrum representing this class; this class is represented with red coloration. c Result of the RDF application for class 3 “digestive system” as overlay over the optical image of the section and an exemplary IR spectrum representing this class; this class is represented with green coloration. d Result of the RDF application for class 4 “other tissue” as overlay over the optical image of the section and an exemplary IR spectrum representing this class; this class is represented with orange coloration