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. Author manuscript; available in PMC: 2023 Feb 15.
Published in final edited form as: Biochemistry. 2022 Jan 24;61(4):294–302. doi: 10.1021/acs.biochem.1c00788

Figure 5. Salt-dependence of intein folding as measured by native Trp fluorescence.

Figure 5.

Native Trp fluorescence was measured at 340 nm via excitation at 295 nm using a Perkin Elmer LS55 fluorescence spectrometer. Gain was set to medium with emission and excitation slit widths of 2.5 nm. Temperature was maintained at 25°C with a Peltier system. Protein GBA was at 10 μM, and protein GBD at 8 μM. Samples were measured in 20 mM HEPES buffer at pH 7.5 at the indicated salt concentrations on the abscissa. Data reported are from two independent trials.