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. 2022 Feb 15;12:2550. doi: 10.1038/s41598-022-06395-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Nest building performance and nest complexity. The development of nest complexity scores differed between age phases in (a) female animals (F(4, 290) = 2.75991, p = 0.02804), but not in (b) male animals (F(4, 290) = 1.07471, p = 0.3692). In male animals, the nest scores in P50 animals exceeded that of P120 animals on the second day (b, #) (F(4, 45) = 2.885, p = 0.0329). Sex differences were evident (c) on the first day in all age groups except for P25 animals (interaction p = 0.3161, age phase p = 0.0205, sex p < 0.0001). In contrast, sex differences in P25 mice became evident (d) only on the last day (interaction p = 0.6905, age phase p = 0.0504, sex p < 0.0001). Linear regression for (a) and (b); one-way ANOVA, followed by Bonferroni multiple comparison tests for #; two-way ANOVA followed by Bonferroni multiple comparison tests for (c) and (d), *p < 0.05, median. Colored dots/lines refer to the respective age groups.