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. 2022 Feb 15;12:2550. doi: 10.1038/s41598-022-06395-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fecal corticosterone metabolite concentrations (FCM) (a) were higher in prepubescence than in pubescence and adulthood in both female and male animals (interaction p = 0.0130, age phase p < 0.0001, sex p < 0.0001). Moreover, concentrations in prepubescent male animals were significantly higher than in all other age groups. Two-way ANOVA, followed by Bonferroni multiple comparison tests, *p < 0.05. Error bars indicate the standard error of the mean (SEM). The age-dependent body weight increase in the different age groups is illustrated in (b). Colored dots refer to the respective age groups. (c) Illustrates an overview of the weekly experimental scheme. B burrowing.