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. 2022 Feb 15;12:2509. doi: 10.1038/s41598-022-06456-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Bead titration in 1,536-well format. (a) Individual dots show mean fluorescence intensity ± SD for D and D + A samples bound to 17 µm beads in matched paired wells of a 1,536-well plate. Number of beads in each well is indicated at top. (1-image/well = 19% of total well area). (b & c) Individual E_FRET values calculated from single D and D + A pair of wells (b), or average of two D and D + A pairs (c). Mean E_FRET ± SD values were calculated for each bead binding condition (n = 192 single D and D + A pair or 96 average of two D and D + A pairs in panels b or c, respectively).