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. 2022 Feb 15;12:2516. doi: 10.1038/s41598-022-06356-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Human induced pluripotent stem cell culture on polyvinyl butyral-based substrate compared with other synthetic chemical substrates. (A) 253G1 and 201B7 colony morphology on PVB, PVA, PEGMA and PNIPAM in TeSR-E8 medium. 253G1 cells show the state on day 4, and 201B7 cells show the state on day 7. TeSR-E8 medium was used for both. (B) 253G1 colony morphology on CPB scaffold during five days in TeSR-E8 medium. All scale bars are 200 µm.