Figure 3.

Temperature effect on peptide-polyvinyl butyral-based coated tissue culturing polystyrene and analysis of detachment of cells on different substrates. (A) 253G1 colony morphology using incubated CPB scaffold at room temperature and 60 ºC for 1 month, (B) Relative absorbance unit of 253G1 after five days of culturing in TeSR-E8 medium after seeding 5 × 10⁴ cells on CPB scaffold. Data are presented as mean ± SD of three independent experiments. (C) Detachment ratio of 253G1 cells cultured on CPB scaffold compared with VTN-N and iMatrix 511. Scale bars is 200 µm. Data are presented as mean ± SD of six independent experiments (*P < 0.05 compared to VTN-N, **P < 0.005 compared to iMatrix511).