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. 2022 Feb 15;12:2516. doi: 10.1038/s41598-022-06356-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Examination colony formation and cell number on CPB scaffold using multiple media. Colony formation of 253G1 and KhES-1 cells after 5 days in Essential 8, TeSR-E8, StemFlex, mTeSR1, or StemFit cultures. CPB scaffold or VTN-N were used as the culture substrate. All scale bars are 200 µm.