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. 2022 Feb 15;13:879. doi: 10.1038/s41467-022-28531-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a–c Oxygen consumption rate (OCR) values after sequential treatment of BMDM with oligomycin (O), FCCP (F) and antimycin + rotenone (A/R) as measured by the Seahorse XF Analyzer at: a prenecrotic (n = 8/group), b necrotic (n = 8/group), and c fibrotic (n = 8/group) phases of disease. OCR values are normalized to cell count. d Lactate levels (normalized to µg of cell lysate protein) in supernatants from WT and mdx BMDM after incubating the cells for 48 h in phenol-red-free RPMI culture media (n = 4/group for prenecrotic, n = 10/group for necrotic, and n = 5/group for fibrotic). e mRNA transcript levels in BMDM (expressed relative to the mean uninjured level represented by the dashed line) obtained from WT mice after acute muscle injury (Injury 1x = injection of cardiotoxin into both tibialis anterior muscles with euthanasia 3 days later; Injury 2x = injection of cardiotoxin into tibialis anterior and gastrocnemius muscles on days 0 and 3, respectively, with euthanasia at day 13) (n = 5/group). f Representative OCR curve from Injury 1x group in e (n = 5/group). Data represent means ± SEM of biologically independent samples from different mice. *P < 0.05 vs. WT (unpaired t-test, two-tailed). See Source Data file for the exact P-values.